NIT Jalandhar

डा बी आर अम्बेडकर राष्ट्रीय प्रौद्योगिकी संस्थान, जालन्धर
Dr B R Ambedkar National Institute of Technology, Jalandhar

Nano-Drop UV-Visible Spectrophotometer

Department: Department of Biotechnology
Concerned Person:
Name Mr. MANOJ KUMAR YADAV
Technician
Department of Biotechnology
Email ID manojky@nitj.ac.in
Mobile No. 9694918662
Location:

BT LAB- 103

Available For:

For Internal Users only

Major Specifications:
  1. Sample volume: 1–2 µL microvolume sample requirement.
  2. Wavelength range: Approximately 190–850 nm.
  3. Measurement: UV–Visible absorbance and full-spectrum analysis.
  4. Pathlength: Automatic pathlength selection for wide concentration ranges.
  5. Analysis time: Rapid measurement within a few seconds.
  6. Concentration range: Suitable for low- to high-concentration samples.
  7. Purity assessment: A260/A280 and A260/A230 ratio measurements.
  8. Data handling: Digital display, spectral visualization, storage, and data export.
Research Potential:
  1. DNA/RNA: Quantification and purity assessment of nucleic acids.
  2. Protein analysis: Protein concentration and UV absorbance measurement.
  3. Microbial studies: OD600 measurement and monitoring of microbial growth.
  4. Enzyme assays: Measurement of enzyme activity and reaction kinetics.
  5. Biochemical assays: Quantification of metabolites and colorimetric assay products.
  6. Nanomaterials: UV–Vis characterization of nanoparticles and nanocomposites.
  7. Genetic Engineering: Quality assessment of samples for PCR, qPCR, sequencing, and cloning.
  8. Biomedical research: Analysis of biomolecules, drug release, and biomaterial–biomolecule interactions.

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Developed by: Computer Centre, Dr. B.R. Ambedkar National Institute of Technology, Jalandhar